4JGP
The crystal structure of sporulation kinase D sensor domain from Bacillus subtilis subsp in complex with pyruvate at 2.0A resolution
Replaces: 4DAHExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-20 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.374, 82.151, 90.678 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.420 - 2.030 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.043 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 36578 | |
<I/σ(I)> | 33.1 | 4.9 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.9 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM CACODYLATE:HCL AND 30% (W/V) PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |