4JGM
Crystal structure of RelB double mutants: Y300F/I335V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.940, 65.940, 65.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.430 - 3.000 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zk9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MERLOT |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.170 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.088 | 0.060 | 0.510 |
| Number of reflections | 3061 | ||
| <I/σ(I)> | 22 | 2.6 | |
| Completeness [%] | 88.0 | 98 | 99.5 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 30% PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
