4JG3
Crystal structure of catabolite repression control protein (crc) from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.659, 74.659, 123.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.590 - 1.800 |
| R-factor | 0.19529 |
| Rwork | 0.194 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jc5 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.009 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.590 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.104 | 0.833 |
| Number of reflections | 37261 | |
| <I/σ(I)> | 13.9 | 1.7 |
| Completeness [%] | 99.5 | 95.2 |
| Redundancy | 11 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.15 | 10%(w/v) PEG1500, 40mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
