4JFQ
A2 HLA complex with L8A heteroclitic variant of Melanoma peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 1-BM-C |
| Synchrotron site | APS |
| Beamline | 1-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-16 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.096, 58.359, 89.432 |
| Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
| Resolution | 84.220 - 1.900 |
| R-factor | 0.2198 |
| Rwork | 0.216 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.797 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 84.220 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 64546 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25 mM MES, 24% PEG 3350, 10 mM NaCl, pH 6.5, vapor diffusion, sitting drop, temperature 291K |
