4JFO
A2 HLA complex with E1A heteroclitic variant of Melanoma peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 1-BM-C |
| Synchrotron site | APS |
| Beamline | 1-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-16 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.283, 63.255, 75.098 |
| Unit cell angles | 81.96, 76.09, 77.98 |
Refinement procedure
| Resolution | 19.925 - 2.110 |
| R-factor | 0.2225 |
| Rwork | 0.219 |
| R-free | 0.29570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.153 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.550 |
| High resolution limit [Å] | 2.110 |
| Number of reflections | 42338 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25 mM MES, 24% PEG 3350, 10 mM NaCl, pH 6.5, vapor diffusion, sitting drop, temperature 291K |
