4JF9
Crystal structure of the wild type red fluorescent protein lanRFP (Branchiostoma Lanceolatum)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 133.634, 133.634, 156.144 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.240 - 2.330 |
| R-factor | 0.1601 |
| Rwork | 0.158 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hvf |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.420 |
| High resolution limit [Å] | 2.330 | 5.030 | 2.330 |
| Rmerge | 0.175 | 0.078 | 0.630 |
| Number of reflections | 34207 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 96.3 | 93.3 | 95 |
| Redundancy | 4.9 | 5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 9 mg/ml of lanRFP in 20 mM Tris pH 8.0, 200 mM NaCl, 5 mM EDTA mixed with an equal amount of reservoir solutions - 1M Na-citrate, 0.1M Tris pH 7.0 , VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
