4JF8
Crystal structure of a TrwG component of type IV secretion system protein from Bartonella birtlesii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12709 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.150, 72.150, 47.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.350 |
| R-factor | 0.15528 |
| Rwork | 0.154 |
| R-free | 0.17514 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bhm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.304 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.390 | |
| High resolution limit [Å] | 1.350 | 6.040 | 1.350 |
| Rmerge | 0.038 | 0.015 | 0.497 |
| Number of reflections | 31529 | ||
| <I/σ(I)> | 34.59 | 103.74 | 2.9 |
| Completeness [%] | 99.7 | 90 | 98.6 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | BabiA.18388.a.B2 PS01693 at 25.7 mg/mL against JCSG+ screen condition A3, 0.2 M citrate, 25% PEG 3350 with 20% ethylene glycol as cryo-protectant, unique puck ID vff2-9, crystal tracking ID 240866a3, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
