4JF8

Crystal structure of a TrwG component of type IV secretion system protein from Bartonella birtlesii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12709
Spacegroup name	P 31 2 1
Unit cell lengths	72.150, 72.150, 47.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.350
R-factor	0.15528
Rwork	0.154
R-free	0.17514
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bhm
RMSD bond length	0.009
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.390
High resolution limit [Å]	1.350	6.040	1.350
Rmerge	0.038	0.015	0.497
Number of reflections	31529
<I/σ(I)>	34.59	103.74	2.9
Completeness [%]	99.7	90	98.6
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	BabiA.18388.a.B2 PS01693 at 25.7 mg/mL against JCSG+ screen condition A3, 0.2 M citrate, 25% PEG 3350 with 20% ethylene glycol as cryo-protectant, unique puck ID vff2-9, crystal tracking ID 240866a3, VAPOR DIFFUSION, SITTING DROP, temperature 289K