4JEU

Crystal Structure of Munc18a and Syntaxin1 with native N-terminus complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-10
Detector	RAYONIX MX-325
Spacegroup name	P 4 21 2
Unit cell lengths	156.775, 156.775, 78.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	110.860 - 3.200
R-factor	0.2042
Rwork	0.199
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c98
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	X-PLOR
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	111.111	111.111	3.370
High resolution limit [Å]	3.200	10.120	3.200
Rmerge	0.109	0.035	0.677
Total number of observations		3775	17494
Number of reflections	16764
<I/σ(I)>	14.8	15.4	1.1
Completeness [%]	100.0	99.8	100
Redundancy	7.1	6.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	298	27% PEG 400, 10 mM EDTA, 10 mM DTT, 224 mM ammonium acetate, and 100 mM sodium acetate , pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K