4JEM
Crystal structure of MilB complexed with cytidine 5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 45.157, 100.386, 71.348 |
| Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
| Resolution | 40.858 - 1.553 |
| R-factor | 0.1876 |
| Rwork | 0.186 |
| R-free | 0.21590 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4jel |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.710 | 1.550 |
| Rmerge | 0.352 | ||
| Number of reflections | 44996 | ||
| <I/σ(I)> | 25.1 | ||
| Completeness [%] | 99.8 | 99 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M magnesium chloride, 18% PEG 8000, 0.1 M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
