4JE9
Crystal structure of an engineered metal-free RIDC1 construct with four interfacial disulfide bonds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.166, 87.788, 96.046 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.020 - 2.120 |
| R-factor | 0.234 |
| Rwork | 0.229 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iq6 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.877 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.023 | 2.240 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.499 | |
| Number of reflections | 13444 | |
| <I/σ(I)> | 17.6 | 1.5 |
| Completeness [%] | 97.5 | 84.4 |
| Redundancy | 5.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 30% methyl-pentanediol, 0.1M Tris pH 8.5, 0.2M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
