4JDX

Structure of the Fluorescence Recovery Protein from Synechocystis sp PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976484
Spacegroup name	P 41 21 2
Unit cell lengths	87.342, 87.342, 230.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.050 - 2.500
R-factor	0.2192
Rwork	0.216
R-free	0.26370
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.061	2.640
High resolution limit [Å]	2.500	2.500
Rmerge		0.012
Number of reflections	31732
<I/σ(I)>		1.7
Completeness [%]	99.8	99.8
Redundancy		7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	10% PEG-3350, 100mM Na-citrate, 2% tacsimate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K