4JDR
Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase from escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 180.000, 180.000, 196.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.300 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.281 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.310 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.115 | 0.561 |
| Number of reflections | 54508 | |
| <I/σ(I)> | 3.4 | 1.4 |
| Completeness [%] | 97.5 | 96.7 |
| Redundancy | 7.48 | 6.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 291 | 2.4M Ammonium sulfate, 0.1M MES, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
