4JDQ

Structure of the Fluorescence Recovery Protein from Synechocystis sp PCC 6803, R60K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00005
Spacegroup name	P 41 21 2
Unit cell lengths	87.406, 87.406, 229.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.089 - 3.520
R-factor	0.2372
Rwork	0.233
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.695
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.090	3.700
High resolution limit [Å]	3.510	3.510
Rmerge		0.015
Number of reflections	11769
<I/σ(I)>		1.6
Completeness [%]	99.6	98
Redundancy		13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	5% PEG-3350, 100mM Na-citrate, 2% tacsimate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K