4JDG
Structure of Tomato Bifunctional Nuclease TBN1, variant N211D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 |
| Unit cell lengths | 115.944, 115.944, 74.713 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.710 - 2.740 |
| R-factor | 0.19748 |
| Rwork | 0.194 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dj4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.413 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.940 | 2.900 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.058 | 0.536 |
| Number of reflections | 9433 | |
| <I/σ(I)> | 16.05 | 1.54 |
| Completeness [%] | 95.6 | 81.5 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M Bis-Tris, 10% PEG 8000, 0.01M 5'-AMP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
