4JDG
Structure of Tomato Bifunctional Nuclease TBN1, variant N211D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | H 3 |
Unit cell lengths | 115.944, 115.944, 74.713 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.710 - 2.740 |
R-factor | 0.19748 |
Rwork | 0.194 |
R-free | 0.26370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dj4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.413 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.940 | 2.900 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.058 | 0.536 |
Number of reflections | 9433 | |
<I/σ(I)> | 16.05 | 1.54 |
Completeness [%] | 95.6 | 81.5 |
Redundancy | 3.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M Bis-Tris, 10% PEG 8000, 0.01M 5'-AMP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |