4JDA
Complex structure of abscisic acid receptor PYL3 with (-)-ABA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 142.738, 142.738, 99.802 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.120 - 2.650 |
| R-factor | 0.24459 |
| Rwork | 0.243 |
| R-free | 0.26728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dsc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.287 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 32032 | |
| Completeness [%] | 95.1 | 70.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 8000, 0.1M Tris-HCl, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
