4JD5
Crystal Structure of Benzoylformate Decarboxylase Mutant L403E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 81.550, 95.790, 137.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.365 - 1.330 |
R-factor | 0.1347 |
Rwork | 0.134 |
R-free | 0.15180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.526 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.7.3_928) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.730 |
High resolution limit [Å] | 1.330 |
Rmerge | 0.089 |
Number of reflections | 122065 |
Completeness [%] | 99.5 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 MM TRIS PH 8.5, 22% V/V PEG 400, 150 MM CACL2, 0.5% V/V MPD, vapor diffusion, hanging drop, temperature 298K |