4JD0
Structure of the inositol-1-phosphate CTP transferase from T. maritima.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-17 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 133.090, 133.090, 43.365 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 115.470 - 1.800 |
| R-factor | 0.1141 |
| Rwork | 0.111 |
| R-free | 0.16606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2QQX |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.354 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.470 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.045 | 0.410 |
| Number of reflections | 38500 | |
| <I/σ(I)> | 25.2 | |
| Completeness [%] | 93.0 | 63.4 |
| Redundancy | 5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.8 2.0 M ammonium phosphate and 100 mM LiCl buffered at pH 8.5 with 100 mM Tris, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
