4JCX

Crystal structure of the Restriction-Modification Controller Protein C.Csp231I OL operator complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	120
Detector technology	CCD
Collection date	2010-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.93930
Spacegroup name	P 61
Unit cell lengths	62.200, 62.200, 147.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.610 - 2.300
R-factor	0.17309
Rwork	0.172
R-free	0.19647
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lfp
RMSD bond length	0.006
RMSD bond angle	1.036
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.610	2.390
High resolution limit [Å]	2.300	2.300
Rmerge	0.111	0.557
Number of reflections	14402
<I/σ(I)>	10.5	3.2
Completeness [%]	99.9	100
Redundancy	6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Protein was dialysed against the buffer containing 0.1 M NaCl, 50 mM TRIS-HCl, pH 8.2, 1 mM DTT and 1 mM EDTA. Crystallisation conditions: 0.2 M ammonium chloride, 0.1 M MES pH 6.0, 20 % (w/v) PEG6K, protein dimer/DNA molar ratio 1:2, protein concentration 1.2 mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 289K