4JCG

Recombinant wild type Nitrosomonas europaea cytochrome c552

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2008
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	P 65 2 2
Unit cell lengths	60.358, 60.358, 109.133
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.409 - 1.630
R-factor	0.1998
Rwork	0.198
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	351c
RMSD bond length	0.016
RMSD bond angle	1.630
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	26.409
High resolution limit [Å]	1.630
Number of reflections	27203
<I/σ(I)>	37.8
Completeness [%]	98.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	19% (w/v) PEG 6000, 0.2 M LiCl, 0.1 M Tris-HCl pH 7.4 at 7-8 mM protein concentration., VAPOR DIFFUSION, HANGING DROP, temperature 277K