4JC4
Crystal structure of Peptidyl-tRNA hydrolase from Pseudomonas aeruginosa at 2.25 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-05-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 62.604, 62.604, 155.392 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.220 - 2.250 |
R-factor | 0.18656 |
Rwork | 0.185 |
R-free | 0.21738 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dhw |
RMSD bond length | 0.027 |
RMSD bond angle | 1.949 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.220 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 16115 | |
<I/σ(I)> | 3.8 | |
Completeness [%] | 98.7 | 88.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% w/v PEG 4000, 5% isopropanol, 100mM HEPES buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |