4JBN

Crystal structure of O-Acetyl Serine Sulfhydrylase from Entamoeba histolytica in complex with Serine acetyl transferase derived tetrapeptide, SPSI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-05-30
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 41
Unit cell lengths	80.515, 80.515, 112.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.470 - 1.870
R-factor	0.1861
Rwork	0.184
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pqm
RMSD bond length	0.022
RMSD bond angle	2.130
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.470	1.917
High resolution limit [Å]	1.870	1.868
Number of reflections	58645
Completeness [%]	94.6	90.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	2.1M AmSO4, 150mM NaCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K