4JBM
Structure of murine DNA binding protein bound with ds DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 111.020, 39.120, 137.877 |
Unit cell angles | 90.00, 110.64, 90.00 |
Refinement procedure
Resolution | 40.973 - 2.218 |
R-factor | 0.2371 |
Rwork | 0.234 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rn2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.367 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.310 |
High resolution limit [Å] | 2.218 | 2.230 |
Number of reflections | 27608 | |
Completeness [%] | 98.4 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.1M sodium malonate, 12%(w/v) polyethylene glycol 3350, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |