4JBE
1.95 Angstrom Crystal Structure of Gamma-glutamyl phosphate Reductase from Saccharomonospora viridis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 265.510, 55.118, 64.246 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 29.730 - 1.950 |
| R-factor | 0.17004 |
| Rwork | 0.168 |
| R-free | 0.20836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o20 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.345 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.068 | 0.548 |
| Number of reflections | 67453 | |
| <I/σ(I)> | 17.3 | 2.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | Protein: 8.3mg/mL, 0.5M Sodium cloride, 0.01M Tris-HCl pH 8.3; Screen: PACT (B11), 0.2M Calcium chloride, 0.1M MES pH 6.0, 20% (w/v) PEG 6000., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
