4JB9
Crystal structure of antibody VRC06 in complex with HIV-1 gp120 core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-10-19 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.244, 68.924, 224.225 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.720 - 2.600 |
R-factor | 0.2496 |
Rwork | 0.247 |
R-free | 0.28740 |
Structure solution method | SAD |
Starting model (for MR) | 3se8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.746 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.093 | 0.375 |
Number of reflections | 29132 | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 92.2 | 57.7 |
Redundancy | 4.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10% PEG 4000, 0.2M sodium acetate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |