4JB6
Structure of Pseudomonas aeruginosa FabF mutant C164Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293.15 |
| Detector technology | CCD |
| Collection date | 2012-08-24 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.073, 104.619, 141.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.377 - 2.400 |
| R-factor | 0.18338 |
| Rwork | 0.179 |
| R-free | 0.27224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b7v Monomer A |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.380 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.168 | 0.542 |
| Number of reflections | 29532 | |
| <I/σ(I)> | 4.06 | 4.9 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 12.1 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Ammonium formate, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
