4JB0
Rhodopseudomonas palustris (strain CGA009) Rp1789 transport protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.970, 70.740, 105.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.240 - 1.910 |
| R-factor | 0.17876 |
| Rwork | 0.176 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 97.5 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 290 | 0.1M sodium acetate, pH 4.5, 25% w/v PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
