4JAB

U/G Wobble Base Pair in a RNA Duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97895
Spacegroup name	H 3
Unit cell lengths	41.681, 41.681, 60.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.930 - 2.230
R-factor	0.15648
Rwork	0.152
R-free	0.22898
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	246d
RMSD bond length	0.009
RMSD bond angle	2.093
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.930	2.310
High resolution limit [Å]	2.230	2.230
Rmerge	0.066	0.330
Number of reflections	1723
Completeness [%]	91.0	90.1
Redundancy	2.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP