4JA9
Rat PP5 apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 50.090, 50.090, 379.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.23931 |
| Rwork | 0.238 |
| R-free | 0.27172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ja7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.991 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.041 | 0.537 |
| Number of reflections | 22932 | |
| <I/σ(I)> | 22.3 | 4.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.2 M Mg(NO3)2, 20% PEG 3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
