4JA3
Partially occluded inward open conformation of the xylose transporter XylE from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 246.880, 74.380, 131.110 |
| Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
| Resolution | 25.260 - 3.800 |
| R-factor | 0.2874 |
| Rwork | 0.285 |
| R-free | 0.31460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.300 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 33439 | |
| Completeness [%] | 82.0 | 29.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 32% PEG 400, 4% 2-methyl-2,4-pentane diol (MPD), 0.2% (w/v) octyl glucoseneopentyl glycol (OGNG), 5 mM CdCl2, 5 mM lutetium acetate, 100 mM temperature 277K, pH 6.0, VAPOR DIFFUSION, SITTING DROP |
