4J8D
Middle domain of Hsc70-interacting protein, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9189 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.290, 126.998, 57.312 |
| Unit cell angles | 90.00, 105.93, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.249 |
| Rwork | 0.246 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j8f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.039 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.499 | 43.036 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.044 | 0.427 | |
| Number of reflections | 19247 | ||
| <I/σ(I)> | 8.3 | 11.4 | 1.8 |
| Completeness [%] | 98.7 | 97.1 | 96 |
| Redundancy | 3.1 | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.15 Na-malonate, pH 7.0, vapor diffusion, hanging drop, temperature 291K |
