4J80
Thermus thermophilus DnaJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00767 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.090, 105.000, 130.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.26697 |
| Rwork | 0.265 |
| R-free | 0.30739 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.561 | |
| Number of reflections | 34862 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 99.2 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 16% PEG6000, 0.1 M succinate/BTP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
