4J7R

Crystal Structure of Chlamydomonas reinhardtii Isoamylase 1 (ISA1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-15
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41 21 2
Unit cell lengths	102.130, 102.130, 488.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.730 - 2.300
R-factor	0.203
Rwork	0.202
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vnc
RMSD bond length	0.007
RMSD bond angle	1.174
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.300	2.300
Rmerge	0.134	0.646
Number of reflections	114033
<I/σ(I)>	11.89	1.53
Completeness [%]	97.5	85.8
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	293	0.2M ammonium citrate tribasic, 14-20% PEG3350, pH 7.0, vapor diffusion, hanging drop, temperature 293K