4J7A
Crystal Structure of Est25 - a Bacterial Homolog of Hormone-Sensitive Lipase from a Metagenomic Library
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 203 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97888, 0.97917, 0.96395, 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 197.758, 95.213, 99.418 |
| Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
| Resolution | 28.555 - 1.492 |
| R-factor | 0.1448 |
| Rwork | 0.143 |
| R-free | 0.16950 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.177 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1063)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.316 | |
| Number of reflections | 285917 | |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 96.2 | 96.2 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.4M sodium malonate pH 7.0, Microbatch, temperature 295K |
