4J72
Crystal Structure of polyprenyl-phosphate N-acetyl hexosamine 1-phosphate transferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9753 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.799, 101.491, 138.313 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.689 - 3.300 |
R-factor | 0.2269 |
Rwork | 0.223 |
R-free | 0.26490 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.193 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.080 | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
Rmerge | 0.071 | ||
Number of reflections | 20890 | ||
<I/σ(I)> | 35.98 | 67.8 | 2 |
Completeness [%] | 99.5 | 96.1 | 97.3 |
Redundancy | 6.5 | 6.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 20-120 mM magnesium acetate, 9 mM nickel chloride, 50 mM sodium cacodylate, pH 5.6, 45% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |