4J6X
Crystal structure of Hfq from Pseudomonas aeruginosa in complex with UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-22 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.580, 73.520, 107.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.222 |
| R-factor | 0.1864 |
| Rwork | 0.183 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.299 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.8.1_1168) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.098 | 0.384 |
| Number of reflections | 24556 | |
| <I/σ(I)> | 13.12 | 3.12 |
| Completeness [%] | 99.2 | 94.01 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 200 mm ammonium sulphate, 200 mm NaCl, 50 mm TRIS-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
