4J6Q
Crystal structure of calcium2+-free wild-type CD23 lectin domain (crystal form G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9687 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.100, 73.180, 62.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.625 - 2.539 |
| R-factor | 0.1789 |
| Rwork | 0.169 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g96 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASES |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.600 |
| High resolution limit [Å] | 2.539 | 2.540 |
| Rmerge | 0.364 | |
| Number of reflections | 4727 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 3.7 M NaCl and 0.1 M citric acid pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
