4J5Y

Crystal structure of Hfq from Pseudomonas aeruginosa in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-22
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	61.450, 73.680, 107.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.574 - 2.095
R-factor	0.1876
Rwork	0.184
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u1t
RMSD bond length	0.007
RMSD bond angle	1.294
Data reduction software	PROTEUM PLUS (PLUS)
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	PHENIX (1.8.1_1168)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.210
High resolution limit [Å]	2.095	2.095
Rmerge	0.091	0.339
Number of reflections	28097
<I/σ(I)>	13.02	3.33
Completeness [%]	95.7	88.44
Redundancy	4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	200 MM ammonium sulphate, 200 MM NaCl, 50 MM TRIS-HCL, PH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K