4J5W
Crystal Structure of the apo-PXR/RXRalpha LBD Heterotetramer Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.260, 109.550, 169.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.536 - 2.800 |
R-factor | 0.2526 |
Rwork | 0.250 |
R-free | 0.29810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1061) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 92.067 |
High resolution limit [Å] | 2.773 |
Number of reflections | 34037 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | 16-20% PEG 8000, 0.1-0.2 M MgCl, 0.1 M bis-tris proprane, 0.02% sodium azide, pH 7.0, vapor diffusion, hanging drop, temperature 277.15K |