4J5Q
TARG1 (C6orf130), Terminal ADP-ribose Glycohydrolase 1, apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.315, 75.372, 35.000 |
| Unit cell angles | 90.00, 100.63, 90.00 |
Refinement procedure
| Resolution | 30.055 - 1.350 |
| R-factor | 0.1323 |
| Rwork | 0.131 |
| R-free | 0.16140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2JYC |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.713 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.099 | 0.085 | 0.445 |
| Number of reflections | 34621 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 98.1 | 93.8 | 96.2 |
| Redundancy | 3.5 | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 277 | 100mM sodium citrate, 20% isopropanol, 20% PEG 4000 , pH 5.6, VAPOR DIFFUSION, temperature 277K |
