4J5G
Crystal structure analysis of Streptomyces aureofaciens ribonuclease Sa T95A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.125, 64.196, 78.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.710 - 1.310 |
| R-factor | 0.11395 |
| Rwork | 0.112 |
| R-free | 0.15001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.580 | 1.320 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.033 | 0.197 |
| Number of reflections | 46925 | |
| <I/σ(I)> | 28.49 | 4.65 |
| Completeness [%] | 93.6 | 80.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | ammonium sulfate 0.8 M, cacodylate buffer 0.1M , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
