4J56
Structure of Plasmodium falciparum thioredoxin reductase-thioredoxin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98696 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.880, 89.180, 151.330 |
| Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
| Resolution | 19.971 - 2.371 |
| R-factor | 0.2383 |
| Rwork | 0.236 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qfb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.207 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Number of reflections | 107823 | |
| <I/σ(I)> | 9.35 | |
| Completeness [%] | 97.9 | 84.7 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 295 | 20% PEG 8000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
