4J4X
Crystal structure of GraVN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 51.289, 96.337, 100.800 |
Unit cell angles | 64.57, 81.72, 85.10 |
Refinement procedure
Resolution | 49.590 - 2.510 |
R-factor | 0.19888 |
Rwork | 0.195 |
R-free | 0.26641 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j4r |
RMSD bond length | 0.013 |
RMSD bond angle | 1.599 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.640 | 2.540 |
High resolution limit [Å] | 2.510 | 2.510 |
Number of reflections | 54500 | |
Completeness [%] | 96.8 | 87.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M MgCl2, 21% PEG 1000, 0.1M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |