4J4S
Triple mutant SFTAVN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.489, 108.479, 221.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.809 - 2.442 |
| R-factor | 0.2111 |
| Rwork | 0.208 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j4r |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 2.500 |
| High resolution limit [Å] | 2.442 | 2.450 |
| Number of reflections | 43028 | |
| Completeness [%] | 97.9 | 73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 3M NaCl, 0.1M NaAc, 0.2M Li2SO4, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
