4J4R
Hexameric SFTSVN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 6 |
| Unit cell lengths | 103.995, 103.995, 48.866 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.031 - 1.900 |
| R-factor | 0.1802 |
| Rwork | 0.179 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ov9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX (phenix.refine: 1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.031 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 21239 | |
| Completeness [%] | 94.5 | 61.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 8% PEG 8000, 0.1 M NaAc, 0.2 M ZnAc2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
