4J40
Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa
Replaces: 3HVBExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 106.251, 106.251, 193.351 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.363 - 2.990 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.23190 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.873 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 3.180 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.062 | 0.494 |
| Number of reflections | 20711 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 99.5 | 97.9 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Na formate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
