4J32
Structure of the effector - immunity system Tae4 / Tai4 from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99981 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.650, 63.650, 365.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.900 - 1.800 |
| R-factor | 0.18291 |
| Rwork | 0.182 |
| R-free | 0.20332 |
| Structure solution method | rigid-body refinement |
| Starting model (for MR) | 4j30 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.102 | 0.661 |
| Number of reflections | 42126 | |
| <I/σ(I)> | 18.7 | 4.7 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 18.1 | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 200 mM tri-sodium citrate pH 6.0, 30 % (v/v) 2-ethoxyethanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
