4J2L

Crystal Structure of AXH domain complexed with Capicua

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-19
Detector	ADSC QUANTUM 315r
Spacegroup name	P 43 2 2
Unit cell lengths	53.500, 53.500, 276.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.230 - 3.150
R-factor	0.236
Rwork	0.236
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j2j
RMSD bond length	0.013
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge		0.663
Number of reflections	8720
<I/σ(I)>		6.2
Completeness [%]	99.9	100
Redundancy		13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6M NaCl, 3%(v/v) glycerol, 16%(w/v) PEG3350, 1mM L-Glutathione, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K