4J29
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR258.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 67.558, 67.558, 48.907 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.885 - 2.100 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.151 | 1.870 |
| Number of reflections | 12646 | |
| <I/σ(I)> | 22.3 | 2.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 27.6 | 28.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP |
