4J24
Estrogen Receptor in complex with proline-flanked LXXLL peptides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.980, 53.490, 131.390 |
| Unit cell angles | 90.00, 120.47, 90.00 |
Refinement procedure
| Resolution | 19.740 - 2.100 |
| R-factor | 0.19174 |
| Rwork | 0.190 |
| R-free | 0.22385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | 0.404 |
| Number of reflections | 67629 | |
| <I/σ(I)> | 23.69 | 5.17 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 277 | 0.2M Magnesium acetate, 20%(w/v) PEG3350, pH 4.5, VAPOR DIFFUSION, temperature 277K |
