4J20
X-ray structure of the cytochrome c-554 from chlorobaculum tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.824, 33.826, 47.333 |
| Unit cell angles | 90.00, 107.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.300 |
| R-factor | 0.1513 |
| Rwork | 0.150 |
| R-free | 0.16996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zon |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.599 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 35354 | |
| Completeness [%] | 99.6 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 3.0M ammonium sulfate, 30mM Zinc chloride, 10% isopropanol, 50mM phosphate buffer, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
